The study aims to identify gene variants that predict skeletal muscle response to both a single bout and 4 weeks of High-Intensity Interval Training (HIIT) in three different training centres. Whilst the lead training and testing centre is located in Victoria University, Melbourne, the lead centre for high throughput genomics will be at Bond University and Queensland University of Technology (QUT).
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The cohort is made up of moderately-trained, healthy participants (all men aged 20-45 years, body mass index ≤ 30 kg/m2). Participants are undergoing similar exercise testing and exercise training in three different laboratories. Dietary habits are assessed by questionnaire and nutritionist consultation. Activity history is assessed by questionnaire and current activity level is assessed by activity monitoring. A number of muscle and blood analyses are to be performed, including genotyping, mitochondrial respiration, transcriptomics, epigenome (methylation status), and enzyme activity.
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\n\nThe Molecular Diagnostics Facility (MDF) was able to provide a comprehensive study design encompassing all aspects of the study. Along with facilitating and advising on the appropriate methodologies, the GRC also advised on the study design and purchase of necessary reagents/assays to perform the study. The GRC will continue to assist in the study to ensure high quality data from template extraction, assay optimisation, assay performance and data collection through to data analysis and interpretation.
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\n\nThe project is ongoing. At this stage, the study design has been finalised, samples collected and assay optimisation is underway.
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\n\n“I am very satisfied with the sequencing service provided by the MDF. The lab staff were very friendly and helpful, and their requirements for sample submission were easy to follow. The rapid turnaround and high data quality meant this was one of the smoothest sequencing projects I have ever worked on.” – Associate Professor Kevin Ashton, Bond University
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\n\nThe Gene SMART (Skeletal Muscle Adaptive Response to Training) study was established to identify gene variants that predict skeletal muscle response to both a single bout and 4 weeks of High-Intensity Interval Training (HIIT) in three different training centres. Whilst the lead training and testing centre is located in Victoria University, Melbourne, the lead centre for high throughput genomics will be at Bond University and Queensland University of Technology (QUT). The cohort is made up of moderately-trained, healthy participants (all men aged 20-45 years, body mass index ≤ 30 kg/m2). Participants are undergoing similar exercise testing and exercise training in three different laboratories.
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Dietary habits are assessed by questionnaire and nutritionist consultation. Activity history is assessed by questionnaire and current activity level is assessed by activity monitoring. A number of muscle and blood analyses are to be performed, including genotyping, mitochondrial respiration, transcriptomics, epigenome (methylation status), and enzyme activity. Currently ~70 participants have finished the study and the overall aim is to train 100 participants. The Gene SMART study also includes baseline and post-training testing and sampling for all participants before, during and after training.
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